knockout mouse

knockout mouse Knockout Mouse 1. Introduction Generally, DNA was first transferred by design into an organism and expressed as protein, therefore the potential of the tool that was being discovered must be found quickly by the scientist. Early experiments were limited to bacteria and viruses, but soon after the field of experiments were enlarged to those on animals and plants. (E.Hill, 2002). This topic, â€Å"knockout mouse† relates to the field of genetic engineering which further delves into a more specific technique called transgenic technology. â€Å"Knockoutscience.com†(2009) analyses that transgenic technology refers to the alteration of a certain genomic DNA of an organism in genetic engineering. As the result, both offspring of a transgenic organism and the parents (homozygous) will share the same genotype. The most common type of transgenic organism used in research is the knockout mouse, thoughknockout rats andknockout rabbits have also been developed. A knockout mouse defines a mouse which is being genetically engineered by turning off one or more genes through a process called gene knockout. â€Å"Genome.gov â€Å"also explains that a knockout mouse is a laboratory mouse in which researchers have inactivated, an existing gene by replacing it or disrupting it with an simulated piece of DNA. The loss of gene activity often causes changes in a mouses phenotype, which includes appearance, activities and other observable physical and biochemical characters. â€Å"Knockoutscience.com† (2009) also elucidates that it has become routine to develop knockout mice with disruptions in specific genes. By observing the resulting phenotype, scientists are able to view the effects of this gene disruption from these knockout mice. It is true to say that the phenotype is a direct result of the gene knockout and can offer evidences as to the biological role of the gene, but rarely the phenotype can also be the result of compensatory or indirect effects of the gene knockout. Sometimes the result obtained in a phenotype can be completely unrelated to the disrupted gene. Additionally, some gene knockouts create alethal phenotypewhere the organism fails to develop in utero, making in vivo studies exceedingly difficult. It was claimed that this technique may help to solve dopamine-related neurological illnesses.(Carol A.T, 1996) The technique allows transgenic animals that lack of a certain gene or its associated protein product to grow. In experiments with mice, researchers were able to knockout the animals dopamine transporters, causing the mice to behave as if they had been given huge doses of cocaine or amphetamine. Walinski.H(2009) states that knockout mice have different way of uses. First, the specific functions of particular genes can be tested and the regulation of these particular genes can be observed. The effects of a particular gene can be determined by examining what is happening in anin vivo model, we are able to determine the effects a particular gene may have. These effects would be impossible to observe in a culture dish. Another useful application of knockout technology is in biomedical research and drug development. Knockout mouse can be used to study the evolution of thousands of genetically based diseases at the molecular level in order to seek for the best medications that act on that gene. For Example, Lili.X and Asok.C (2005 ) both agree that Duffy positive and Duffy knockout mice have revealed both human malaria parasite Plasmodium vivax and mouse malaria parasite Plasmodium yoelii by using parasite invasion. Furthermore, the knockout technology may lead to the discovery of the next generation of blockbuster therapies for curing numerous diseases based on novel targets from the human genome. 2. Background Genetics Timeline for the key events in the history of knockout mouse 1900 Japanese fancy mice became mutant resources for mouse genetics. 1915- The first verterbrate linkage (mapping) was discovered between albino (c) and pink-eyed dilution (p) loci in the mouse. 1923- Discovery of X-ray induced mutations in mouse before the phenomenon was confirmed in fruit-flies. 1980- Specific-locus tests were conducted extensively in the mouse with various chemical mutagens, including N-ethyl-N-nitrosourea (ENU). 1981-1991- knockout mice are established. 1981- The first embryo stem (ES) cell was identified in the mouse. Martin Evans and Matt Kaufman in Cambridge, U.K., isolate mouse embryonic stem cells, which can develop into the full range of tissues. 1982-Transgenic mouse technology was established through the generation of the â€Å"giant mouse† mutant. 1985- Introduction of the Cre-loxP system by Brian Sauer act as temporal control of transgenic gene expression. 1987- Mario Capecchis team at the University of Utah describes a method for making knockout mice, as does Oliver Smithiess group at the University of Wisconsin. 1989- First knockout mouse was made by combining ES cell and gene-targeting technologies. 2007- International Knockout Mouse Consortium was organized and the Banbury II meeting was held in Brussels, Belgium. Nobel Prize for Physiology and Medicine was awarded for the development of mouse knockout techonologies. 3. Genetic Technique There are several method to produce knockout mouse, such as gene deletion, homologous recombination method, pronuclear microinjection and gene targeting. However, only gene targeting will be mentioned herein. Gene Targeting is the elimination or alteration of a genes function. One of the advantages of gene targeting is a mutant allele can be mended by substituting a wild-type allele over the mutant one in its normal chromosomal location, and such technique known as gene replacement. In this way, both position effect and the DNA rearrangements associated with ectopic insertion can be prevented, as a single replication of the gene is inserted in its normal chromosomal environmental. (Griffiths.A and Susan R.Wessler,etc, 2008). 4. Social Issues Recently, the evaluation of animal and human welfare as it may be affected by biotechnology is becoming a hot issue. The lack of an conscience and the information of the processes involved is one of the most important fact. ( Marie.B, 1997) Marie.B (1997) also states that the moral evaluation process is complicated by the fact that many techniques and developments in biotechnology are appropriate for patent. Some of the biotechnologists are reluctant to reveal appropriate information is understandable. Therefore, education concerning transgenic animal care and utilize is indeed very importance, involving the careful consideration of the reasons for manipulating the genome of any organism as genetic engineering is a dangerous and sensitive social issue. ( Marie.B, 1997) Pros and Cons of Knockout Mouse Advantages Disadvantages Provides important clues about what that gene normally does because human share many genes with mice. (Genome.gov, 2009) Limitation of the utility of knockout mice as models of human disease. (Walinski.H, 2004) Gives better understanding and observation of the characteristics of knockout mice. ( Genome.gov, 2009) The lack of adult mice limits studies to embryonic development and makes it more difficult to determine a genes function in relation to human health. ( Genome.gov, 2009) Gives information that can be used to better understand how a similar gene may cause or contribute disease in human. ( Genome, 2009) The gene that being examined might serve a different function in adults than in developing embryos, giving a false information. (Genome, 2009) Useful in studying and modeling different kinds of cancer, obesity, heart disease, diabetes, arthritis, substance abuse, anxiety, aging and Parkinson disease. Fails to produce an observable change in a mouse or may even produce a different characteristics from those observed in humans in which the same gene is inactivated. ( Genome.gov, 2009) Offers a biological context in which drugs and other therapies can be developed and tested. Producing custom knockout mice is very expensive. It can be from 3000 to as much as 30,000 (Walinski.H, 2004) Useful in drug development and helps to discover the next generation of blockbuster therapies for curing numerous diseases based on novel targets from the humane genome. (Walinski.H, 2004) The cost of equipping and maintaining such a facility is usually very high. ( Walinski.H, 2009) 5.0: Conclusion In conclusion, it is clear that knockout mouse offer a lot of benefits for us. Therefore, a thorough discussion of biotechnology issues is needed, as concurrence must be practiced as to protect transgenic animals. The field of transgenic animal biotechnology is likely to rise as the techniques develop further and will link to more applications by using many more animal species. Thus, it is important that the welfare and ethical concerns must continue to evolve. (Marie.B, 1997) In short, technology essentials together with thoughtful ethical decision-making are equally important to maintain the balance of living creatures. 6.0: Bibliography * Connor A.B, 2007, Schematic and Time Line for the Generation of Knockout Mice, Viewed 25 January 2010, http://web.mit.edu/ki/facilities/transgenic/services/timeline-complete.pdf * Davidson, NC 28036, Homologous Recombination Knockout Mouse, Department of Biology, Davidson College, viewed 15 January 2010, http://www.bio.davidson.edu/Courses/genomics/method/homolrecomb.html. * Eisen, Eugene J, 2005, Mouse in Animal Genetics and Breeding Research, viewed 25 January 2010, http://site.ebrary.com/lib/sunway/docDetail.action?docID=10173945p00=knockout+mouse * Garagna, S, 2004, Mouse Genetics after the Mouse Genome, Viewed 25 January 2010, http://site.ebrary.com/lib/sunway/docDetail.action?docID=10070304p00=knockout+mouse * Genome.gov, 2009, Knockout Mice, Viewed 25 January 2010, http://www.genome.gov/12514551 * Genoway ( 2006), Gene deletion, conditional knockout mouse, viewed 11 february 2010, http://www.genoway.com/conditional_knock_out_mouse.htm * Griffiths.A.J, Wessler.S.R, Lewontin.R.C and Carroll.S.B (2008), Introduction to genetic analysis, W.H,Freeman and Company, England. * â€Å"Google docs†, 2010, Gene targeting construct, Viewed 25 January 2010, http://74.125.153.132/search?q=cache:j6mj7wNqd7gJ:tmc.ctrl.ucla.edu/targeting.pdf+history+knockout+mousehl=engl=my * Hudson, 2010, Taconic enters marketing pact with Transposagen Bio for knockout rat models, viewed 25 January 2010, http://www.pharmabiz.com/article/detnews.asp?articleid=53683sectionid= * Marie.B (1997) Transgenic animlas, viewed 11 february 2010,http://www.ccac.ca/en/Current/Current_Transgenic.htm * National Center for Biotechnology Information, 2007, What is transgenic technology? Viewed 15 January 2010, http://www.knockoutscience.com/ * Research Resources, n.d., Transgenic Knockout Mouse, viewed 25 January 2010, http://www.cellmigration.org/resource/komouse/protocols/mouse_timeline.pdf * â€Å"University of California†, n.d., Transgenic and Gene Targeting Core, viewed 25 January 2010, http://cancer.ucsd.edu/tgm/genetargeting.asp#timeline * Table, http://www.knockoutmouse.org/ * Tamminga, Carol A. Molecular biology, I: a mouse knockout. (molecular technique called gene knockout which allows the development of animals that lack a certain gene and its associated protein product).American Journal of Psychiatry153.11 (1996): 1387.Academic OneFile. Web. 6 Feb. 2010. . * Twyman. R, 2003, Knockout Mice: The inactivation of a specific gene in a mouse, viewed on 15 January 2010, http://genome,wellcome.ac.uk/doc_WTD021038.html * Walinski.H, 2004, Studying Gene Function: Creating Knockout Mice, viewed on 25 January 2010, http://www.scq.ubc.ca/studying-gene-function-creating-knockout-mice/ * Walter E.Hill. , 2002, Genetic Engineering, Taylor Francis, Biddles LTd, Guildford and Kings Lynn, Great Britain. * â€Å"Wikipedia†, 2009, Knockout Mouse, viewed on 15 January 2010, http://en.wikipedia.org/wiki/Knockout_mouse * Xu, Lili, and Asok Chaudhuri. Plasmodium yoelii: A differential fluorescent technique using Acridine Orange to identify infected erythrocytes and reticulocytes in Duffy knockout mouse.Experimental Parasitology110.1 (2005): 80+.Academic OneFile. Web. 6 Feb. 2010. . * Y. Gondo (2008), Trends in large-scale mouse mutagenesis: from genetics to functional genomics. Nat. Rev. Genet. 9(10):803-810., http://www.nature.com/scitable/content/Knockout-mice-timeline-6638351, Viewed On 11 February 2010.

Acoustics :: acoustic acoustics sound

The concert hall is one of man's greatest joys; enjoying a fine symphony composed by the worlds greatest, performed by a world class orchestra. For months afterwards, the borgoise attendees will speak of the energy of the conductor, the orchestra responding to him, the power of it all. The crescendoes, the diminuendos, the stark silences between movements, the clarity of the soloist above the rest of the orchestra. Little do they know that were it not for the meticulous consideration of the architect at construction time, the patrons would not feel that they were getting their money's 'worth.' The architect is tapping into the world of acoustics; in this case, achieving the best possible sonic experience through differing techniques : different materials, certain shapes of rooms, sound traps, etcetera. Acoustics and sound, like so many physics branches, starts out relatively simple and quickly becomes a gigantic headache. Massive amounts of planning must go into a large structure such as a concert hall, a dance hall. Even small structures, such as the phonebooth, have hours of careful planning put into their design, in order to achieve the desired effect with the varied sound sources. This page is designed to inform the reader in the basics of acoustics, using the theory behind acoustics and the examples of the concert hall and the dancehall. Technically, the definition of 'sound' is a pressure wave in an elastic medium. This means that our pressure wave can be present in air, water, wood, steel... many different solids as well as air (and liquid). One of the most common units of measurement of sound is intensity, or the rate at which sound energy is being transmitted into the medium. (low sound intensity would be something like somebody clapping between movements, high sound intensity would be the applause after Beethoven's 9th) The unit of intensity is the decibel, or db. We use a logarithmic scale to measure these, as it is difficult to understand the range of the decibel in linear terms. Other units of measurement are the period and the amplitude of the wave. As we see in the graphic, we have the graph of time versus sound pressure, with our little wave traversing it. The amplitude is the highest value the graph reaches before descending again, while the period is the amount of time it takes for the wave to complete a full cycle. Other terms of note for the diagram are compression, which is represented by the parts of the graph in the positive region of sound pressure, and rarefraction, which is represented by negative pressure.

Carrie Chapter Eleven

Billy offered her a ride home from school one afternoon a week later and she accepted. He was what the other kids called a white-soxer or a machine-shop Chuck. Yet something about him excited her and now, lying drowsily in this illicit bed (but with an awakening sense of excitement and pleasurable fear), she thought it might have been his car – at least at the start. It was a million miles from the machine-stamped, anonymous vehicles of her fraternity dates with their ventless windows, fold-up steering wheels, and vaguely unpleasant smell of plastic scat covers and windshield solvent. Billy's car was old, dark, somehow sinister, the windshield was milky around the edges, as if a cataract was beginning to form. The seats were loose and unanchored. Beer bottles clicked and rolled in the back (her fraternity dates drank Budweiser; Billy and his friends drank Rheingold), and she had to place her feet around a huge, grease-clotted Craftsman toolkit without a lid. The tools inside were of many different makes, and she suspected that many of them were stolen. The car smelled of oil and gas. The sound of straight pipes came loudly and exhilaratingly through the thin floorboards. A row of dials slung under the dash registered amps, oil pressure, and tach (whatever that was). The back wheels were jacked and the hood seemed to point at the road. And of course he drove fast. On the third ride home one of the bald front tyres blew at sixty miles an hour, the car went into a screaming slide and she shrieked aloud, suddenly positive of her own death. An image of her broken, bloody corpse, thrown against the base of a telephone pole like a pile of rags, flashed through her mind like a tabloid photograph. Billy cursed and whipped the fuzz-covered steering wheel from side to side. They came to a stop on the left-hand shoulder, and when she got out of the car on knees that threatened to buckle at every step, she saw that they had left a looping trail of scorched rubber for seventy feet Billy was already opening the trunk, pulling out a jack and muttering to himself. Not a hair was out of place. He passed her, a cigarette already dangling from the corner of his mouth. ‘Bring that toolkit, babe.' She was flabbergasted. Her mouth opened and closed twice, like a beached fish, before she could get the words out. ‘I-I will not! You almost k-you-almost-you crazy bastard! Besides, it's dirty!' He turned around and looked at her, his eyes flat. ‘You bring it or I ain't taking you to the fuckin fights tomorrow night.' ‘I hate the fights!' She had never been, but her anger and outrage required absolutes. Her fraternity dates took her to rock concerts, which she hated. They always ended up next to someone who hadn't bathed in weeks. He shrugged, went back to the front end, and began jacking. She brought the toolkit, getting grease all over a brandnew sweater. He grunted without turning around. His teeshirt had pulled out of his jeans, and the flesh of his back was smooth, tanned, alive with muscles. It fascinated her, and she felt her tongue creep into the corner of her mouth. She helped him pull the tyre of the wheel, getting her hands black. The car rocked alarmingly on the jack, and the spare was down to the canvas in two places. When the job was finished and she got back in, there were heavy smears of grease across both the sweater and the expensive red skirt she was wearing. ‘If you think-‘ she began as he got behind the wheel. He slid across the seat and kissed her, his hands moving heavily on her, from waist to breasts. His breath was redolent of tobacco; there was the smell of Brylcreem and sweat. She broke it at last and stared down at herself, gasping for breath. The sweater was blotted with road grease and dirt now. Twenty-seven-fifty in Jordan Marsh and it was beyond anything but the garbage can. She was intensely, almost painfully excited. ‘How are you going to explain that?' he asked, and kissed her again. His mouth felt as if he might be grinning.' ‘Feel me,' she said in his car. ‘Feel me all over. Get me dirty.' He did. One nylon split like a gaping mouth. Her skirt, short to begin with, was pushed rudely up to her waist. He groped greedily, with no finesse at all. And something – perhaps that, perhaps the sudden brush with death – brought her to a sudden, jolting orgasm. She had gone to the fights with him. ‘Quarter to eight,' he said, and sat up in bed. He put on the lamp and began to dress, His body still fascinated her. She thought of last Monday night, and how it had been. He had (no) Tune enough to think of that later, maybe, when it would do something for her besides cause useless arousal. She swung her own legs over the edge of the bed and slid into gossamer panties. ‘Maybe it's a bad idea,' she said, not sure if she was testing him or herself. ‘Maybe we ought to just get back into bed and-‘ ‘It's a good idea,' he said, and a shadow of humour crossed his face. ‘Pig blood for a pig.' ‘What?' ‘Nothing. Come on. Get dressed.' She did, and when they left by the back stairs she could feel a large excitement blooming, like a rapacious and night-flowering vine, in her belly. From My Name Is Susan Snell (p. 45): You know, I'm not as sorry about all of it as people seem to think I should be. Not that they say it right out; they're the ones who always say how dreadfully sorry they are. That's usually just before they ask for my autograph. But they expect you to be sorry. They expect you to get weepy, to wear a lot of black, to drink a little too much or take drugs. They say things like: ‘Oh, it's such a shame. But you know what happened to her-‘ and blah, blah, blah. But sorry is the Kool-Aid of human emotions. It's what you say when you spill a cup of coffee or throw a gutterball when you're bowling with the girls in the league. True sorrow is as rare as true love. I'm not sorry that Tommy is dead any more. He seems too much like a daydream I once had. You probably think that's cruel, but there's been a lot of water under the bridge since Prom Night. And I'm not sorry for my appearance before The White Commission. I told the truth – as much of it as I knew. But I am sorry for Carrie. They've forgotten her, you know. They've made her into some kind of a symbol and forgotten that she was a human being, as real as you reading this, with hopes and dreams and blah, blah, blah. Useless to tell you that, I suppose. Nothing can change her back now from something made out of newsprint into a person. But she was, and she hurt. More than any of us probably know, she hurt. And so I'm sorry and I hope it was good for her, that prom. Until the terror began, I hope it was good and fine and wonderful and magic †¦ Tommy pulled into the parking lot beside the high school's new wing, let the motor idle for just a second, and then switched it of. Carrie sat on her side of the seat, holding her wrap around her bare shoulders. It suddenly seemed to her that she was living in a dream of hidden intentions and had just become aware of the fact. What could she be doing? She had left Momma alone. ‘Nervous?' He asked, and she jumped. ‘Yes.' He laughed and got out. She was about to open the door when he opened it for her. ‘Don't be nervous,' he mid. ‘You're like Galatea.' ‘Who?' ‘Galatea. We read about her in Mr Evers' class. She turned from a drudge into a beautiful woman and nobody even knew her.' She considered it. ‘I want them to know me,' she said finally. ‘I don't blame you. Come on.' George Dawson and Frieda Jason were standing by the Coke machine. Frieda was in an orange tulle concoction, and looked a little like a tuba. Donna Thibodeau was taking tickets at the door along with David Bracken. They were both National Honour Society members, part of Miss personal Gestapo, and they wore white slacks and red blazers – the school colours. Tina Blake and Norma Watson were handing out programmes and seating people inside according to their chart Both of them were dressed in black, and Carrie supposed they thought they were very chic, but to her they looked like cigarette girls in an old gangster movie. All of them turned to look at Tommy and Carrie when they came in, and for a moment there was a stiff, awkward silence. Carrie felt a strong urge to wet her lips and controlled it. Then George Dawson said: ‘Gawd, you look queer, Ross.' Tommy smiled. ‘When did you come out of the treetops, Bomba?' Dawson lurched forward with his fists up, and for a moment Carrie felt stark terror. In her keyed-up state, she came within an ace of picking George up and throwing him across the lobby. Then she realized it was an old game, often played, well-loved. The two of them sparred in a growing circle. Then George, who had been tagged twice in the ribs, began to gobble and yell:- ‘Kill them Congs! Get them Gooks! Pongee sticks! Tiger cages!' and Tommy collapsed his guard, laughing. ‘Don't let it bother you,' Frieda said, tilting her letteropener nose and strolling over. ‘If they kill each other, I'll dance with you.' ‘They look too stupid to kill,' Carrie ventured. ‘Like dinosaurs.' And when Frieda grinned, she felt something very old and rusty loosen inside her. A warmth came with At. Relief. Ease. ‘Where'd you buy your dress?' Frieda asked. ‘I love it.' ‘I made it.' ‘Made it?' Frieda's eyes opened in unaffected surprise. ‘No shit!' Carrie felt herself blushing furiously. ‘Yes I did. I †¦ I like to sew. I got the material at John's in Andover. The pattern is really quite easy.' ‘Come on,' George said to all of them in general. ‘Band's gonna start.' He rolled his eyes and went through a limber, satiric buck-and-wing. ‘Vibes, vibes, vibes. Us Gooks love them big Fender viyyybrations.' When they went in, George was doing impressions of Flash Bobby Pickett and mugging. Carrie was telling Freida about her dress, and Tommy was grinning, hands stuffed in his pockets. Spoiled the lines of his dinner jacket Sue would be telling him, but fuck it, it seems to be working. So far it was working fine. He and George and Frieda had less than two hours to live. From The Shadow Exploded (p. 132): The White Commission's stand on the trigger of the whole affair – two buckets of pig blood on a beam over the stage – seems to be overly weak and vacillating, even in light of the scant concrete proof. If one chooses to believe the hearsay evidence of Nolan's immediate circle of friends (and to be brutally frank, they do not seem intelligent enough to lie convincingly), then Nolan took this part of the conspiracy entirely out of Christine Hargensen's hands and acted on his own initiative †¦ He didn't talk when he drove; he liked to drive. The operation gave him a feeling of power that nothing could rival, not even fucking. The road unrolled before them in photographic blacks and whites, and the speedometer trembled just past seventy. He came from what the social workers called a broken home; his father had taken off after the failure of a badly managed gas-station venture when Billy was twelve, and his mother had four boyfriends at last count. Brucie was in greatest favour right now. He was a Seagram's 7 man. She was turning into one ugly bag, too. But the car: the car fed him power and glory from its own mystic lines of force. It made him someone to be reckoned with, someone with mana. It was not by accident that he had done most of his balling in the back seat. The car was his slave and his god. It gave, and it could take away. Billy had used it to take away many times. On long, sleepless nights when his mother and Brucie were fighting, Billy made popcorn and went out cruising for stray dogs. Some mornings he let the car roll, engine dead, into the garage he had constructed behind the house with its front bumper dripping. She knew his habits well enough by now and did not bother making conversation that would simply be ignored anyway. She sat beside him with one leg curled under her, gnawing a knuckle. The fights of the cars streaking past them on 302 gleamed softly in her hair, streaking it silver. He wondered how long she would last. Maybe not long after tonight. Somehow it had all led to this, even the early part, and when it was done the glue that had held them together would be thin and might dissolve, leaving them to wonder how it could have been in the first place. He thought she would start to look less like a goddess and more like the typical society bitch again, and that would make him want to belt her around a little. Or maybe a lot. Rub her nose in it. They breasted the Brickyard Hill and there was the high school below them, the parking lot filled with plump, glistening daddies' cars. He felt the familiar gorge of disgust and hate rise in his throat. We'll give them something (a night to remember) all right. We can do that. The classroom wings were dark and silent and deserted; the lobby was lit with a standard yellow glow, and the bank of glass that was the gymnasium's east side glowed with a soft, orangey light that was ethereal, almost ghostly. Again the bitter taste, and the urge to throw rocks. ‘I see the lights, I see the party fights,' he murmured. ‘Huh?' She turned to him, startled out of her own thoughts. ‘Nothing.' He touched the nape of her neck. ‘I think I'm gonna let you pull the string.' Billy did it by himself, because he knew perfectly well that he could trust nobody else. That had been a hard lesson, much harder than the ones they taught you in school, but he had learned it well. The boys who had gone with him to Henty's place the night before had not even known what he wanted the blood for. They probably suspected Chris was involved, but they could not even be sure of that. He drove to the school minutes after Thursday night had become Friday morning and cruised by twice to make sure it was deserted and neither of Chamberlain's two police cars was in the area. He drove into the parking lot with his lights off and swung around in back of the building. Further back, the football field glimmered beneath a thin membrane of ground fog. He opened the trunk and unlocked the ice chest. The blood had frozen solid, but that was all right. It would have the next twenty-four hours to thaw. He put the buckets on the ground, then got a number of tools from his kit. He stuck them in his back pocket and grabbed a brown bag from the seat. Screws clinked inside. He worked without hurry, with the easeful concentration of one who is unable to conceive of interruption. The gym where the dance was to be held was also the school auditorium, and the small row of windows looking toward where he had parked opened on the backstage storage area. He selected a flat tool with a spatulate end and slid it through the small jointure between the upper and lower panes of one window. It was a good tool. He had made it himself in the Chamberlain metal shop. He wriggled it until the window's slip lock came free. He pushed the window up and slid in. It was very dark. The predominant odour was of old paint from the Dramatic Club canvas flats. The gaunt shadows of Band Society music stands and instrument cases stood around like sentinels. Mr Downer's piano stood in one corner. Billy took a small flashlight out of the bag and made his way to the stage and stepped through the red velvet curtains. The gym floor, with its painted basketball lines and highly varnished surface, glimmered at him like an amber lagoon. He shone his light on the apron in front of the curtain. There, in ghostly chalk fines, someone had drawn the floor silhouette of the King and Queen thrones which would be placed the following day. Then the entire apron would be strewn with paper flowers †¦ why, Christ only knew. He craned his neck and shone the beam of his light up into the shadows. Overhead, girders crisscrossed in shadowy lines. The girders over the dance floor had been sheathed in crepe paper, but the arm directly over the apron hadn't been decorated. A short draw curtain obscured the girders up there, and they were invisible from the gym Floor. The draw curtain also hid a bank of lights that would highlight the gondola mural. Billy turned off the flashlight, walked to the left-hand edge of the apron, and mounted a steel-runged ladder bolted to the wall. The contents of his brown bag, which he had tucked into his shirt for safety, jingled with a strange, hollow jolliness in the deserted gymnasium. At the top of the ladder was a small platform. Now, as he faced outward toward the apron, the stage flies were to his right, the gym itself on his left. In the flies the Dramatic Club props were stored, some of them dating back to the 1920s. A bust of Pallas, used in some ancient dramatic version of Poe's ‘Me Raven,' stared at Billy with blind, floating eyes from atop a rusting bedspring Straight ahead, a steel girder ran out over the apron. Lights to be used against the mural were bolted to the bottom of it. He stepped out on to it and walked effortlessly, without fear, over the drop. He was humming a popular tune under his breath. The beam was inch-thick with dust, and he left long shuffling tracks. Halfway he stopped, dropped to his knees, and peered down. Yea. With the help of his light he could make out the chalk lines of the apron directly below. He made a soundless whistling. (bombs away) He X'd the precise spot in the dust, then beam-walked back to the platform. No one would be up here between now and the Ball; the lights that shone on, the mural and on the apron where the King and Queen would be crowned (they'll get crowned an right) were controlled from a box backstage. Anyone looking up from directly below would be blinded by those same lights. His arrangements would be noticed only if someone went up into the flies for something. He didn't believe anyone would. It was an acceptable risk. He opened the brown bag and took out a pair of Playtex rubber gloves, put them on, and then took out one of two small pulleys he had purchased yesterday. He had gotten them at a hardware store in Boxford, just to be safe. He popped a number of nails into his mouth like cigarettes and got the hammer. Still humming around his mouthful of nails, he fixed the pulley neatly in the corner above the platform. Beside it he fixed a small eyehole screw. He went back down the ladder, crossed backstage, and climbed another ladder not far from where he had come in. He was in the loft – sort of a catchall school attic. Here there were stacks of old yearbooks, moth-eaten athletic uniforms, and ancient textbooks that had been nibbled by mice. Looking left, he could shine his light over the stage flies and spotlight the pulley he had just put up. Turning right, cool night air played on his face, from a vent in the wall. Still humming, he took out the second pulley and nailed it up. He went back down, crawled out the window he had forced, and got the two buckets of pig blood. He had been about his business for a half hour, but it showed no signs of thawing. He picked the buckets up and walked back to the window, silhouetted in the darkness like a farmer coming back from the first milking. He lifted them inside and went in after. Beam-walking was easier with a bucket in each hand for balance. When he reached his dust-marked X, he put the buckets down, peered at the chalk marks on the apron once more, nodded, and walked back to the platform. He thought about wiping the buckets on his last trip out to them – Kenny's prints would be on them, Don's and Steve's as well – but it was better not to. Maybe they would have a little surprise on Saturday morning. The thought made his lips quirk. The last item in the bag was a coil of jute twine. He walked back out to the buckets and tied the handles of both with running slipknots. He threaded the screw, then the pulley. He threw the uncoiling twine across to the left, and then threaded that one. He probably would not have been amused to know that, in the gloom of the auditorium, covered and streaked with decades-old dust, grey kitties flying dreamily about his crow's nest hair, he looked like a hunched, half-mad Rube Goldberg intent upon creating the better mousetrap. He piled the slack twine on top of a stack of crates within reach of the vent. He climbed down for the last time and dusted off his hands. The thing was done. He looked out the window, then wriggled through and thumped to the ground. He closed the window, reinserted his jimmy, and closed the lock as far as he could. Then he went back to his car. Chris said chances were good that Tommy Ross and the White bitch would be the ones under the buckets; she had been doing a little quiet promoting among her friends That would be good, if it happened. But, for Billy, any of the others would be all right too. He was beginning to think that it would be all right if it was Chris herself. He drove away. From My Name Is Susan Snell (p. 48): Carrie went to see Tommy the day before the prom. She was waiting outside one of his classes and he said she looked really wretched, as if she thought he'd yell at her to stop hanging around and stop bugging him. She said she had to be in by eleven-thirty at the latest, or her momma would be worried. She said she wasn't going to spoil his time or anything, but it wouldn't be fair to worry her momma.

The Battle Of The Civil War Essay - 924 Words

Of the number of different battles of the Civil War, there are only three that will be mentioned in this essay. The purpose is to expand the knowledge of the three battles and provide a better understanding behind the reasoning of the Civil War. In addition, the wars, which consisted of bloody combats and unique strategies, provided a settlement for who controls the United States of American and its future. The three main battles that changed the course of history are the following: the Battle of Bull Run, the Western Theater, and the Virginia Front of 1862. The result of the war, of course, was the 13th Amendment, which abolished slavery, permitted the Union to gain control over the South, caused over 600,000 casualties, and provided a victory for millions of African American slaves. Indeed, the war also resulted in Lincoln’s final draft of the Emancipation Proclamation. The two armies, the Confederates and the Union, fought their first major battle in northern Virginia. It w as known that the Union had over 30,000 men under General Irvin McDowell’s control just outside of Washington. The Confederate, on the other hand, were located approximately thirty miles away at Manassas. The smaller army was under P. G. T. Beauregard. The first clash between the two happened on July 21, 1861, when General Irvin McDowell led his inexperienced army toward Manassas. Thus, the First Battle of Bull Run, or also called First Battle of Manassas, began. Toward the end of the battle,Show MoreRelatedThe Battle Of The Civil War1127 Words   |  5 Pagesshaken by the Civil War because some states wanted to separate themselves from this unity that had lasted for approximately 100 years. The American Civil War is a historic affair and one of the bloodiest battles in history of the US (Engle, Krick, 2003). It is considered one of the bloodstained happenings in the history of the US because more than 600, 000 people died. A majority of this number were soldiers. This fat al battle happened between the years 1861 to 1865. The Civil War emerged becauseRead MoreThe Battle Of The Civil War Essay1424 Words   |  6 PagesThroughout the years 1861 to 1865 the American Civil War took place and it is known as Americas bloodiest war. The cause of the Civil war was the debate between free states and slave states over the government trying to prohibit slavery. In 1860 Abraham Lincoln became president of the United States and ran on the platform to keep slavery out of the new unclaimed territories of the United States. Once Lincoln became president seven southern states seceded from the Union ultimately creating the ConfederateRead MoreThe Battle Of The Civil War1666 Words   |  7 PagesThe Civil war was one of the biggest turning points in United States history. The nation’s failure to compromise on the subject of slavery led to an outbreak of war. Battles such as Gettysburg, Vicksburg, and Shiloh led to what we know of today as a free nation. Great technological advancements in weaponry led to mass c asualties among both north and south, often making death inevitable. Those who bravely served and those who were forced to serve lived a life of fear, starvation, and commitment toRead MoreThe Battle Of The Civil War1191 Words   |  5 PagesWhen the Civil War started both sides thought the war would be over by Christmas. But little did they know this small war would turn into the deadliest war in American history. The Civil War was fought between the Confederacy and the Union. The Confederacy was consisted of Southern states that did not agree with the views of the Union states. After years of sectional differences in the United States between the north and south, tension between the states grew and a war between the north and southRead MoreThe Battle Of The Civil War1073 Words   |  5 PagesThe Civil War was a war that was thought to would have lasted no longer than ninety days. After all was said and done, the war lasted more than four years and claimed tens of thousands of li ves of both The Union and The Confederate States. There were several battles fought on Northern and Southern soil, battles that forced bloody mortal combat between brothers in the fight for equality amongst all men. It tested the strength of the foundation that the United States was built upon. The Civil War wasRead MoreThe Battle Of The Civil War960 Words   |  4 PagesHave you ever wondered why the Civil War happened or why it was so substantial to American History? What was the battle that changed it all; that made it the history it is today? Many people have made speculations on the causes of the war and what the major turning point was, but cannot really narrow it down. In James McPherson’s Crossroads of Freedom: Antietam he points out the pivotal moments in American history that led up to and changed the Civil War, so that the reader can get a better insightRead MoreThe Battle Of The Civil War1197 Words   |  5 PagesMarianna, FL. The battle will be virtually unkno wn in the history of the Civil War, but its success marked the longest successful incursion of Union troops into Florida (Cox, 2014). This raid had two goals; the first was to rescue the reported Union prisoners and the second was to liberate and enlist any slaves that were found along the route (Calvin, 2014). The prisoners were not located, but more than 600 slaves were liberated along their route (Cox, 2014). The subsequent battle did not last longRead MoreThe Battle Of The Civil War1163 Words   |  5 Pageshad already decided to secede from the Union. Those 7 states started the Confederate States of America, run by their own President, Jefferson Davis. This same issue was the catalyst for the Civil War which started on April 12, 1861. One of the major and well-known battles of the Civil War was the Battle of Gettysburg. It was on this backdrop that Abraham Lincoln delivered a speech that is â€Å"universally recognized by historians and scholars alike as the most famous and most important speech everRead MoreThe Battle Of The Civil War1233 Words   |  5 PagesIt is evident that the Civil War helped shape America into the country it is today. The strong factional division -based on slavery- between the Union and the Confederacy guaranteed certain achievements of both sides. Much of what we consider history today remains a part of the outcome from these multiple bloody battles. Activist John Muir of the Civil War-era stated, â€Å"the traces of war are not only apparent on the broken fields, burnt fences, mills, and woods ruthlessly slaughtered, but also onRead MoreThe Battle Of The Civil War1133 Words   |  5 Pagesfierce battles were fought to secure the rights and freedom of both men and women, irrespective of their religion, gender, and race. Of all of the battles, the American Civil War stands out. Divided into the Union and the Confederacy, the American civil war broke out after the southern states seceded due to slavery that spread in many parts of the country. The Civil War began in the year 1861 and ended four years later. The end result was the Union becoming victorious in 1865. The Civil War was a

Facebook s Influence On Social Media - 1549 Words

With rapid advancements in technology, social media has become more accessible to people allowing them to spend a great amount of time on social media. Types of social media include Facebook, Twitter, Snapchat, Instagram, and Tumblr. However, it wasn’t long before Facebook became one of the most used types of social media compared to others with about 1.15 billion users (Crowell 11). Reasons as to why Facebook has become more popular include that it allows people to do many things such as posting a status, picture, or a video. It allows people to communicate with others all across the world and gives people the ability to make new friends. Facebook also allows you to chat with friends along with playing games. Although it allows for such†¦show more content†¦With Facebook being used frequently, many have taken on research to see what type of people are using Facebook and why it is so popular among users of social network sites. In the article, â€Å"Self-Affirmation Underlies Facebook Use,† Toma and Jeffrey believe that the reason for the popularity of Facebook â€Å"can be understood through the fulfillment of ego needs† (321). Toma and Jeffrey recognize that other reasons as to why social network cites may be popular could be due to â€Å"procrastinating, gossiping, relieving boredom, or expressing narcissistic drives† (321). However, in the article, they propose that what motivates people to use social network sites are â€Å"†¦that [it] allow users to (a) craft self-presentations that reveal core aspects of their self-concept, such as social affiliations and treasured characteristics, and (b) highlight social connections with friends and family, satisfy fundamental ego needs regarding desired self-images† (Toma and Jeffrey 321). In other words, Toma and Jeffrey are saying that one of the reasons that people turn to social media, in this case Facebook, is because Facebook provides a way for people to satisfy t heir need of self-affirmation by both looking at others profiles and creating their own. They created two studies to test their hypothesis and found that in their first study, Facebook users who spent at least five minutes on their profiles were

Plate Tectonics And Its Effects On The World - 1718 Words

Maybe the most ideal approach to see how Pinnacles started is to envision that you are viewing a video shot somewhere in the range of 60 million years prior completion at the present time. Not wishing to see something that is milleniums-long, you accelerate the activity by keeping your finger on the quick forward catch. Regardless. At last regardless you know the story all around ok to rehash it. Plate Tectonics At the point when the video starts, the mainlands have the structure we know today; the vast majority of the world s extraordinary mountain reaches are set up, and the Pacific Ocean is so far inland that a large portion of California s beach front mountains don t exist. Yet, they are going to be conceived - on account of something many refer to as plate tectonics. Geologists trust that the world s hull is isolated into huge plates that fit together like the bits of a mammoth jigsaw riddle. These plates are thought to move persistently, for the most part on a level plane. As per geologic hypothesis, the plates meet in no less than three ways, and two of these are imperative to our story: subduction zones (where one plate moves underneath the other), and change limits (where the two plates grind past each other). At some point around sixty million years prior, when our video starts, the lighter-weight North American Plate started abrogating or subducting the denser Farallon plate, and as it did, its external edge went about as a bulldozer, gathering upShow MoreRelatedThe Theory Of Plate Tectonics1487 Words   |  6 PagesThe theory of plate tectonics says the lithosphere is split into different plates that move around on top of the surface of the Earth. Though it is just a theory geologic time, ocean trenches, seafloor spreading, mountains, vol canoes, and earthquakes are all examples of evidence that support the theory of plate tectonics. Geologic time shows sediment patterns in different layers of the Earth. Geologic time is separated in three main periods called the Cenozoic Era, the Paleozoic Era, and the MesozoicRead MoreEffects on Motion and Plate Techtonics Essay examples776 Words   |  4 PagesUniversity of Phoenix Material Effects of the Motions of Tectonic Plates Worksheet From Visualizing Earth Science, by Merali, Z., and Skinner, B. J, 2009, Hoboken, NJ: Wiley. Copyright 2009 by Wiley. Adapted with permission. Part 1 This diagram was similar to one Alfred Wegener drew to show the distribution of continents 300 million years ago. His theory of continental drift was met with skepticism in the scientific community. 1. Describe three forms of evidence Wegener usedRead MoreEssay about The Creation of the Ocean Floor818 Words   |  4 PagesSea floor spreading and plate boundaries are not a common subject to anyone outside of a scientific or marine biology lab. The purpose of this paper is to identify plate boundaries. Compare and contrast ideas behind plate tectonics and the theory of the continental drift. There are many types of plate boundaries. Each one is unique in its own way. Below three of the primary types are described in detail. There are several natural events that occur as a direct result of plate boundary interaction. Read MoreTectonic Plates Worksheet852 Words   |  4 PagesUniversity of Phoenix Material Effects of the Motions of Tectonic Plates Worksheet From Visualizing Earth Science, by Merali, Z., and Skinner, B. J, 2009, Hoboken, NJ: Wiley. Copyright 2009 by Wiley. Adapted with permission. Part 1 This diagram was similar to one Alfred Wegener drew to show the distribution of continents 300 million years ago. His theory of continental drift was met with skepticism in the scientific community. [pic] 1. Describe three forms of evidence WegenerRead More Tectonic Plates and its Effects on the Physical Face of the Earth1476 Words   |  6 PagesTectonic Plates and its Effects on the Physical Face of the Earth For millions of years, tectonic plates have been determinate of changes in the physical face of the earth, and they continue to do so today. These massive plates move underneath the surfaces of the oceans and the continents, producing earthquakes, volcanoes and uplifts. This paper will discuss the composition, movement and history of tectonic plates, the theory of plate tectonics and its history, and tectonic plates affect theRead MoreTectonic Plates and Their Movement655 Words   |  3 PagesThe world as we know it today is the result of a process that has taken million of years and is still ongoing today. The movement of the earth is considered to be one of the most interesting and at the same time debated subjects science deals with particularly because there have been numerous attempts to try to explain the creation of the earth, its morphology, and current evolution. The tectonic plates and their movement are the basis of seismology which tries to explain the way in which the mountainsRead MoreInc1 Science Rint Task 11255 Words   |  6 PagesScientific Concepts and the Natural World RINT Task 1 Western Governors University Free Powerpoint Templates Page 1 The Earth’s Plate Tectonics James Hutton was the first scientist to address the Earth was millions of years old, as well as alive and is continuously being formed. Charles Lyell popularized uniformitarianism, and believed the Earth was being shaped by slow moving forces. Alfred Wegener introduced the foundation for the theory of continental drift. Wegener was one of theRead MoreThe Theory Of Plate Tectonics And Continental Drift950 Words   |  4 PagesThe concept of plate tectonics is that the lithosphere, which is the higher layers of the Earth’s surface, is separated into a number of plates that float independently over the mantle and along the boundaries that initiate the development of new crust, the building of mountains, and the seismic movement that triggers earthquakes (Geosystems 340). Scientists have discovered that the landforms and geographic regions we know and live on today were formed when plate tectonics shift and create movementRead MoreEssay on Plate Tectonics Explained1398 Words   |  6 Pagesdensity of a fluid is related to its temperature. Hot rocks lower in the mantle are less dense than the cooler rocks above. The hot rocks rise and the cooler rocks sink because of gravity. Convection currents are thought to be the driving mechanism of plate movement. Convection currents cause convergent and divergent movements. When the rising part of the convection current rises it causes the upper mantle to move upward and in a lateral direction. This causes the mantle to split and new material to riseRead MoreThe East African Rift Valley1424 Words   |  6 PagesSeafloor spreading when new oceanic crust is being created where two tectonic plates are diverging from one another. One of the largest examples of this is the East African Rift Valley. â€Å"In simple terms, a rift can be thought of as a fracture in the earth s surface that widens over time, or more technically, as an elongate basin bounded by opposed steeply dipping normal faults† (geology.com). The East African Rift is a 3000 km long feature that began developing millions of years ago. The rift travels

Testing Template and Amendments for Ensuring Maximum Efficiency

Question: Manage Business Document Design And Development? Answer: Introduction Creation as well as utilization of well-designed documents is one of the cornerstones of modern office along with business environment. It is considered necessary for people to get entry in the world of business in order to have some level of competence in terms of generating documents and forms utilizing common software packages. At present days business, most of the business documents are created as well as maintained electronically utilizing a range of organizational and commercial software packages. Hence, it is important to be familiar with the procedures such as databases, desktop publishing, word processing as well as spreadsheets that are discussed in the present paper. In addition, essential skills for good administrator and the methods used by the organization for printing as well as presenting the documents of business are discussed in the study. Assessment 1 Procedures for automating the information entry, quality of document design, storage, output and production In order to ensure documents like business letters, reports, newsletter, memos and forms, it is required to maintain consistent style and layout (Wasson, 2015). The following design as well as production criteria is generally utilized and to be adhered by the professionals to produce documents. Design and production criteria Maintain proper format in line with brand name of the organization. Set quality standard dictating everything from the language utilized to print size of text Maintain effective content protection by specification of formatting as well as inclusion of legal notices and statements Comply with ethical principles, anti-discrimination legislation and privacy laws Creation of achieve protocol and consisting of version control for document retrieval On the other hand, specification of designing the format can be explained as followed. Size and location of logo of the organization. Example: top right of document and 4*3 cm. A proper subject line The type and size of the font Justification of the text Size of heading and subheadings The utilization of white space Laguna and Marklund (2013) stated that there are several aspects for considering at the time of storing documents. Documents need to be stored as template on central server of the organization. In addition, it is important to ensure the file paths structured logically as well as encounter requirements of the organization. Output is the format, which is needed by the organization. Some of the organizations need that clients retrieve information and notification in a particular format. For an instance, clients need to be informed by letter as email in order to encounter legal needs. Present and future information technology capability Conducting detailed investigation of the companys present as well as future technology needs impact on the production of documents procedure. There are two separate cafes in the organization. Main site of the production is generated and delivered to smaller site. Majority of the email has capabilities from PC (Cassidy, 2016). Information technology requirements have major changes that need to enhance the communication between sites as well as running the sites. Types of documents Pollock, Wick and Jefferson (2015) mentioned that mail merger letter has several requirements like several addresses, fill-in the area for free-flow of the texts, formatted text, embedded graphics and linking the texts from different documents. On the other hand, business email requirements are formatted text, embedded graphics or text, link-in text from different documents. The prescribed font styles along with formatting and address book is included in this. Spreadsheets have replaced by paper based calculations of accounting in the organization. The powerful software program can be utilized through providing address of the customers. Documentation standards and design tasks The documentations are generally varied in every organization. Software functions can be highly useful. Letter templates are required by most of the organizations. On contrary, managers of the organization seek to get information regarding function. The developer tools allow developing sophisticated programs within the application of software. Signature protection detects if the users have reviewed as well as approved the document. The form fields have limits of requirements for data entry and verify the data entry. However, merge criteria defines the criteria for merging the documents and avoids duplication of documentation. Standard formats and templates suitable requirements of the organization Style guides processes as well as templates that are complement to each other in production of document. A style can guide and manually describes the proper utilization of style as well as paragraph settings and terminology to be used and has desired for spelling, grammar along with punctuation and the process of covering presentation of the document. It is important to check whether there is information on copyright (Lhe Legner, 2014). In addition, anti-virus, may be an issue during running of the macros. Document templates improves readability and appearance It is important to meet the standards as well as designs tasks for the audience and information needs of each document. The documents need to be protected from the unauthorized access and need to be utilized for protecting the sections of document from altering through general users. It also allows the data entry in the unprotected cells within the spreadsheet. The sections breaks allow distinctive styles to be utilized with various sections. Chang (2016) asserted that there are scopes for providing extra information regarding elements of the document and additional information to the users of templates. However, it is required to conceal during printing of the letters. The reference file names require changing with the file names. On the other hand, there may be version conflict with older ones. Feedback of the users is helpful to understand the change and make amendments essential for having maximum efficiency as well as quality of presentation. Complex technical aspects of software It is important to evaluate complicated technical functions of software in order automate the aspects of standard document production. The needs of particular documents have changed in broader external changes that can affect validity as well as currency of macro. The changes in the organization like change of name, address as well as trading names need to be included in this process. However, changes in the billing and invoices methods along with other difficulties may arise from the anti-virus software at the time of running macros. Match requirements of the document with software functions In document presentation of an organization, it is required to match the requirements with the software functions. The company requires detecting the documents and it development process along with functions of software (Vincent, Li Blandford, 2014). For an example, it is required to check whether the printer has adequate capability for printing quality images. In addition, checking of accessing digital camera inside the organization and copying the documents to DVD for storage purposes need to be checked by the company. Testing of macros Laguna and Marklund (2013) stated that the running of anti-virus application need not to generate issues as there is several clicks performing to accept the macros. These are duplicated on the workstation running the macro. Use of different software is utilizing no applications of anti-virus. Therefore, it is required to define the macros on the computers that are running no antivirus. In addition, it is important to test the macros in order to encounter the needs of every document according to the standard of document. Davenport (2013) commented that essential skills for good office administrator in terms of business documentation are required to identify in the case scenario. Administrative skills are associated with running business as well as keeping the office in the organized way. The skills are required for variety of tasks that has ranges from office assistants to the secretary office managers. On the other hand, being an administrator, it is important to make proper documentation of business. In this skill, it is required to have effective communication skill that helps coordinate with the members of the organization. However, it is required to have computing skill, use of several documentation software, planning, and problem solving skill as well as accuracy are required for developing business Effective use of methods for printing and presenting business documents give competitive advantage. Printings as well as presenting documents of business are vital aspects of a business organization (Caetano et al. 2016). It generally comes with effective delivery of business messages. Hence, using correct methods can enhance understanding as well as assist to get motivation. In present business organization, printer is used for printing brochures, fliers, catalogs, reports as well as letters. On the other hand, in order to present the documentation, the company follows power point presentation software. In addition, charts and graphs provide visual pop to the numbers of presenting. Methods used by others would be helpful to adopt better method in future. After talking to friend, relative and teacher, it is known that they use printer for the purposes of printing their documents. Trkman (2013) commented that there are several types of printers available such as dot-matrix printers, daisy-wheel printers, line printers, ink-jet printer and laser printer. Out of these, ink-jet printers are used by them. Standing in front of group as well as speaking is a difficult task to achieve (Lehoux et al. 2014). Hence, it is important to use effective methods for presenting the documents. Charts and graphs are presented by them using Microsoft excel. However, presentation is achieved by them using projector. It helps to get in touch of various people and present ideas and documentation to them. It is important to identify the mistakes done by Monica. In order to modify the documents in a variety of software applications, Monica opened them at a time. She started to working on them. However, she left the applications. Hence, running all applications at a time may slow the processing speed of the organization. In addition, as the three applications were running at a time, the RAM of the system became block and display banks screen and went blank. Hence, it was required to back up the data after a short interval of time or enabling automatic back up feature of Microsoft word in order to avoid such unwanted situations. On contrary, CD writes drive, zip drive is useful for backing up data from computer. It is vital to identify the work practices that generate errors and damage in works as well. In the present case scenario Monica needs to more aware in terms of making documentation. On the other hand, it is required to take essential steps such as formation of effective planning and achieve work according to that. Monica needs to back up data in regular interval. USB drive is one of the useful mediums for taking back up of data. In addition, using software applications effectively according to the guidelines can be helpful to Monica to modify the applications properly. Anti-virus can be useful for Monica to prevent unwanted break down of the system. In the present case scenario, Monica faced unwanted breakdown of the system. As she opens three applications at a while, the system became slower than usually it runs. Hence, it is required to take actions that would prevent the situation. As consequences of this situation, the applications were suddenly closed along with breakdown of the system. In addition, Monica was unable to modify the documents and recover the data as well. In order to solve the issue, she needs to make proper planning and use the application one by one. Along with these, it is required to take back up of data while modifying the tasks in different software applications. Conclusion From the above discussion, it can be concluded that producing business document is one of the important aspects for an organization in order to develop effective result. In this aspect, use of proper technology and software application acts an important role for the particular organization. In addition, selection of layout as well as style of publication ensure consistency in effective development of the design and make documentation. Clarification of format as well as style would be enhanced with the help of this process. References Caetano, A., Silva, A. R., Tribolet, J., Neves, J., Sinogas, P. (2016, June). The Modify Project: Combined Business and System Modeling for Adaptable Enterprise Computing System Design. InAtas da Conferncia da Associao Portuguesa de Sistemas de Informao(Vol. 1, No. 1). Cassidy, A. (2016).A practical guide to information systems strategic planning. CRC press. Chang, J. F. (2016).Business process management systems: strategy and implementation. CRC Press. Davenport, T. H. (2013).Process innovation: reengineering work through information technology. Harvard Business Press. Laguna, M., Marklund, J. (2013).Business process modeling, simulation and design. CRC Press. Lehoux, P., Daudelin, G., Williams-Jones, B., Denis, J. L., Longo, C. (2014). How do business model and health technology design influence each other? Insights from a longitudinal case study of three academic spin-offs.Research Policy,43(6), 1025-1038. Lhe, J., Legner, C. (2014). Overcoming implementation challenges in enterprise architecture management: a design theory for architecture-driven IT Management (ADRIMA).Information Systems and e-Business Management,12(1), 101-137. Pollock, R. V., Wick, C. W., Jefferson, A. (2015).The six disciplines of breakthrough learning: How to turn training and development into business results. John Wiley Sons. Trkman, P. (2013). Increasing process orientation with business process management: Critical practices.International Journal of Information Management,33(1), 48-60. Vincent, C. J., Li, Y., Blandford, A. (2014). Integration of human factors and ergonomics during medical device design and development: It's all about communication.Applied ergonomics,45(3), 413-419. Wasson, C. S. (2015).System Engineering Analysis, Design, and Development: Concepts, Principles, and Practices. John Wiley Sons